Hi Yield® Small DNA Fragments Extraction Kit

The Hi Yield^® Small DNA Fragments Extraction Kit is a Gel Extraction Kit and PCR Cleanup Kit in 1 convenient system optimized to recover or concentrate 40-200 bp DNA fragments from agarose gel, PCR, or other enzymatic reactions.

Chaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.

Typically, recoveries are up to 90% for Gel Extraction and up to 95% for PCR Cleanup.

The eluted DNA is ready for use in PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation.

Advantages

• Purify small DNA in as little as 10 minutes!
• Up to 95% recovery of small DNA fragments
• Optimized for 40 bp–200 bp DNA fragment recovery
• Sample Size: up to 300 mg (agarose gel slice), up to 100 μl (PCR products)
• Spin Columns: glass fiber membrane optimized for both gel extraction and PCR cleanup of small DNA fragments
• Storage: dry at room temperature for up to 2 years